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human burkitt s lymphoma cell line  (ATCC)


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    ATCC human burkitt s lymphoma cell line
    Human Burkitt S Lymphoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+burkitt+s+lymphoma+cell+line/Raji%3B+Burkitt's+Lymphoma%3B+Human/us12570961-468-10-23
    Average 96 stars, based on 650 article reviews
    human burkitt s lymphoma cell line - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Methods and compositions for T-cell coculture potency assays and use with cell therapy products
    Article Snippet: The terms “Daudi cell” or “Daudi cell line” mean a human Burkitt's lymphoma cell line, which is available from multiple vendors, including the American Type Culture Collection (Manassas, VA, USA), and derivatives, variants, modifications, and progeny thereof.

    Article Title: Therapy-enhancing glucan
    Article Snippet: Study Design Cell Lines: Human Burkitt's lymphoma cell line, Daudi, and Hodgkin's disease (HD) cell lines Hs445 and RPMI 6666 were purchased from American Type Culture Collection (Rockville, Md.).

    Article Title: A novel chimeric antigen receptor (CAR) system using an exogenous protease, in which activation of T cells is controlled by expression patterns of cell-surface proteins on target cells
    Article Snippet: A human T-cell acute lymphoblastic leukemia cell line, Jurkat, a human Burkitt's lymphoma cell line, Raji, and a human chronic myelogenous leukemia cell line, K562 were purchased from American Type Culture Collection (ATCC) and maintained in RPMI-1640 media with 10% fetal bovine serum (FBS).

    Article Title: Methods and compositions for T-cell coculture potency assays and use with cell therapy products
    Article Snippet: The terms “U937 cell” or “U937 cell line” mean a human histiocytic lymphoma cell line, which is available from multiple vendors, including the American Type Culture Collection (Manassas, VA, USA), and derivatives, variants, modifications, and progeny thereof.

    Article Title: Synthesis and biological evaluation of novel 2-arylvinyl-substituted naphtho[2,3- d ]imidazolium halide derivatives as potent antitumor agents
    Article Snippet: Two series of novel 2-arylvinyl-naphtho[2,3-d]imidazol-3-ium iodide derivatives and 2-arylvinyl-naphtho[2,3-d]imidazol-3-ium bromide derivatives were designed and synthesized by the structural combination of YM155 with stilbenoids.. All compounds were tested for anti-proliferative activity against PC-3, A375 and HeLa human cancer cell lines.. Two of the compounds were selected for further investigation: 12b, which showed potent cytotoxicity against the three tested cell lines with IC50 values in the range of 0.06-0.21 μM, and 7l, which displayed excellent selectivity for PC-3 cells with an IC50 of only 22 nM.

    Cell Culture:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..

    Protein Extraction:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..

    Saline:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..

    Lysis:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..

    Protease Inhibitor:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..

    Incubation:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..

    Centrifugation:

    Article Title: Hycanthone derivatives and Primaquine derivatives for use in the prevention and/or the treatment of disorders associated to gammaherpesvirus
    Article Snippet: .. Materials and Methods Cell Culture and Protein Extraction: BC-3, an HHV-8 positive lymphoblastic cell line (ATCC CRL-2277) and RAMOS, an human Burkitt's lymphoma cell line (ATCC CRL-1596), were routinely maintained in RPMI 1640 medium supplemented with 10% fetal heat-inactivated bovine serum (FBS), and antibiotics (Sigma Chemical Co., St. Louis, Mo.) in a 5% CO2 incubator at 37° C. To prepare whole cell extracts, the cells were washed with cold phosphate-buffered saline twice and lysed in lysis RIPA buffer containing 10 mM Tris-HCl, pH 8.0, 140 mM NaCl, 1% Triton X-100, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.1% SDS, supplemented with protease inhibitor mixture (1 mM PMSF, 10 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate), (ThermoFisher Scientific) at 4° C. for 30 min. After incubation, the mixture was pipetted five or six times to disperse the cells followed by centrifugation at 14,000 rpm at 4° C. for 10 min. ..



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to profile tumor and microenvironment from ovarian cancer patient samples and evaluate cell-based therapy using in vitro killing assays

    doi: 10.1016/j.xpro.2025.103742

    Figure Lengend Snippet:

    Article Snippet: Human Burkitt’s lymphoma cell line RAJI , ATCC , CAT#CCL-86, RRID: CVCL_0511.

    Techniques: Blocking Assay, Activation Assay, Virus, Recombinant, Cell Culture, Saline, Red Blood Cell Lysis, Cell Isolation, Staining, Transfection, Plasmid Preparation, Software, Flow Cytometry, Imaging, Sterility, Cell Counting